plasmid rp4 Search Results


oatp rp3  (ATCC)
90
ATCC oatp rp3
Oatp Rp3, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+rp4/Escherichia+coli+DH10B+with+plasmid+pCMV-Sport2%2C+OATP-RP4/us07795392-193-10-6
Average 90 stars, based on 1 article reviews
oatp rp3 - by Bioz Stars, 2026-10
90/100 stars
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conjugative plasmid rp4  (Addgene inc)
90
Addgene inc conjugative plasmid rp4
Figure 2. Lower frequency but higher relative importance of genR transfer via phage delivery (relative to conjugation) occurs at lower bacterial concentration. (a) Transfer frequency was determined after 1 h of conjugation and phage delivery assays in minimal medium with 3.0 g/L glucose and shaken at an Re of 5 × 104. The genR donor (E. coli DH5α harboring plasmid <t>RP4</t> for conjugation assays or λ phage for phage delivery assays) and recipient (E. coli MG1655) were mixed at a 1:1 ratio. Frequency determined by the plate counting method is presented as bars with the detection limit (10−6) indicated by the horizontal dashed line; orange dots represent the frequency determined by qPCR analysis. Asterisks (*) indicate significant differences (p < 0.05) between conjugation and phage delivery frequency based on Student’s t-test. “N.D.” refers to “not detected”. (b) Upregulation of conjugation-related genes (traI, traJ, and trbAp) and phage delivery-related genes (lamB and malT) at lower bacterial concentration (twofold gene expression change). The top X-axis depicts decreasing bacterial concentration (CFU/mL). The expression level at a bacterial concentration of 108 CFU/mL was used as the control. Error bars depict ± one standard deviation from the mean of at least three independent replicates.
Conjugative Plasmid Rp4, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+rp4/RP4-8+(Plasmid+%2379813)/pm37101581-28-0-6
Average 90 stars, based on 1 article reviews
conjugative plasmid rp4 - by Bioz Stars, 2026-10
90/100 stars
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plasmid rp4  (Balzer GmbH)
90
Balzer GmbH plasmid rp4
Figure 2. Lower frequency but higher relative importance of genR transfer via phage delivery (relative to conjugation) occurs at lower bacterial concentration. (a) Transfer frequency was determined after 1 h of conjugation and phage delivery assays in minimal medium with 3.0 g/L glucose and shaken at an Re of 5 × 104. The genR donor (E. coli DH5α harboring plasmid <t>RP4</t> for conjugation assays or λ phage for phage delivery assays) and recipient (E. coli MG1655) were mixed at a 1:1 ratio. Frequency determined by the plate counting method is presented as bars with the detection limit (10−6) indicated by the horizontal dashed line; orange dots represent the frequency determined by qPCR analysis. Asterisks (*) indicate significant differences (p < 0.05) between conjugation and phage delivery frequency based on Student’s t-test. “N.D.” refers to “not detected”. (b) Upregulation of conjugation-related genes (traI, traJ, and trbAp) and phage delivery-related genes (lamB and malT) at lower bacterial concentration (twofold gene expression change). The top X-axis depicts decreasing bacterial concentration (CFU/mL). The expression level at a bacterial concentration of 108 CFU/mL was used as the control. Error bars depict ± one standard deviation from the mean of at least three independent replicates.
Plasmid Rp4, supplied by Balzer GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+rp4/plasmid+rp4/pm15228524-176-17-19
Average 90 stars, based on 1 article reviews
plasmid rp4 - by Bioz Stars, 2026-10
90/100 stars
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donor host in bacterial conjugation (for rp4 orit/orir6k derivative plasmids)  (BioResource International Inc)
90
BioResource International Inc donor host in bacterial conjugation (for rp4 orit/orir6k derivative plasmids)
Figure 2. Lower frequency but higher relative importance of genR transfer via phage delivery (relative to conjugation) occurs at lower bacterial concentration. (a) Transfer frequency was determined after 1 h of conjugation and phage delivery assays in minimal medium with 3.0 g/L glucose and shaken at an Re of 5 × 104. The genR donor (E. coli DH5α harboring plasmid <t>RP4</t> for conjugation assays or λ phage for phage delivery assays) and recipient (E. coli MG1655) were mixed at a 1:1 ratio. Frequency determined by the plate counting method is presented as bars with the detection limit (10−6) indicated by the horizontal dashed line; orange dots represent the frequency determined by qPCR analysis. Asterisks (*) indicate significant differences (p < 0.05) between conjugation and phage delivery frequency based on Student’s t-test. “N.D.” refers to “not detected”. (b) Upregulation of conjugation-related genes (traI, traJ, and trbAp) and phage delivery-related genes (lamB and malT) at lower bacterial concentration (twofold gene expression change). The top X-axis depicts decreasing bacterial concentration (CFU/mL). The expression level at a bacterial concentration of 108 CFU/mL was used as the control. Error bars depict ± one standard deviation from the mean of at least three independent replicates.
Donor Host In Bacterial Conjugation (For Rp4 Orit/Orir6k Derivative Plasmids), supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+rp4/donor+host+in+bacterial+conjugation++for+rp4+orit+orir6k+derivative+plasmids+/pmc10135574-2-8-16
Average 90 stars, based on 1 article reviews
donor host in bacterial conjugation (for rp4 orit/orir6k derivative plasmids) - by Bioz Stars, 2026-10
90/100 stars
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rp4 plasmid  (INCF)
90
INCF rp4 plasmid
Figure 2. Lower frequency but higher relative importance of genR transfer via phage delivery (relative to conjugation) occurs at lower bacterial concentration. (a) Transfer frequency was determined after 1 h of conjugation and phage delivery assays in minimal medium with 3.0 g/L glucose and shaken at an Re of 5 × 104. The genR donor (E. coli DH5α harboring plasmid <t>RP4</t> for conjugation assays or λ phage for phage delivery assays) and recipient (E. coli MG1655) were mixed at a 1:1 ratio. Frequency determined by the plate counting method is presented as bars with the detection limit (10−6) indicated by the horizontal dashed line; orange dots represent the frequency determined by qPCR analysis. Asterisks (*) indicate significant differences (p < 0.05) between conjugation and phage delivery frequency based on Student’s t-test. “N.D.” refers to “not detected”. (b) Upregulation of conjugation-related genes (traI, traJ, and trbAp) and phage delivery-related genes (lamB and malT) at lower bacterial concentration (twofold gene expression change). The top X-axis depicts decreasing bacterial concentration (CFU/mL). The expression level at a bacterial concentration of 108 CFU/mL was used as the control. Error bars depict ± one standard deviation from the mean of at least three independent replicates.
Rp4 Plasmid, supplied by INCF, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+rp4/rp4+plasmid/pmc07919528__MSB___17___e9913___s001-92-22-69
Average 90 stars, based on 1 article reviews
rp4 plasmid - by Bioz Stars, 2026-10
90/100 stars
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plasmid rp4  (Struve Labs)
90
Struve Labs plasmid rp4
Figure 2. Lower frequency but higher relative importance of genR transfer via phage delivery (relative to conjugation) occurs at lower bacterial concentration. (a) Transfer frequency was determined after 1 h of conjugation and phage delivery assays in minimal medium with 3.0 g/L glucose and shaken at an Re of 5 × 104. The genR donor (E. coli DH5α harboring plasmid <t>RP4</t> for conjugation assays or λ phage for phage delivery assays) and recipient (E. coli MG1655) were mixed at a 1:1 ratio. Frequency determined by the plate counting method is presented as bars with the detection limit (10−6) indicated by the horizontal dashed line; orange dots represent the frequency determined by qPCR analysis. Asterisks (*) indicate significant differences (p < 0.05) between conjugation and phage delivery frequency based on Student’s t-test. “N.D.” refers to “not detected”. (b) Upregulation of conjugation-related genes (traI, traJ, and trbAp) and phage delivery-related genes (lamB and malT) at lower bacterial concentration (twofold gene expression change). The top X-axis depicts decreasing bacterial concentration (CFU/mL). The expression level at a bacterial concentration of 108 CFU/mL was used as the control. Error bars depict ± one standard deviation from the mean of at least three independent replicates.
Plasmid Rp4, supplied by Struve Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+rp4/plasmid+rp4/10__1186_slash_s42269___022___00956___0-282-23-5
Average 90 stars, based on 1 article reviews
plasmid rp4 - by Bioz Stars, 2026-10
90/100 stars
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plasmids rp4 and r68.45  (Federation of European Neuroscience Societies)
90
Federation of European Neuroscience Societies plasmids rp4 and r68.45
Figure 2. Lower frequency but higher relative importance of genR transfer via phage delivery (relative to conjugation) occurs at lower bacterial concentration. (a) Transfer frequency was determined after 1 h of conjugation and phage delivery assays in minimal medium with 3.0 g/L glucose and shaken at an Re of 5 × 104. The genR donor (E. coli DH5α harboring plasmid <t>RP4</t> for conjugation assays or λ phage for phage delivery assays) and recipient (E. coli MG1655) were mixed at a 1:1 ratio. Frequency determined by the plate counting method is presented as bars with the detection limit (10−6) indicated by the horizontal dashed line; orange dots represent the frequency determined by qPCR analysis. Asterisks (*) indicate significant differences (p < 0.05) between conjugation and phage delivery frequency based on Student’s t-test. “N.D.” refers to “not detected”. (b) Upregulation of conjugation-related genes (traI, traJ, and trbAp) and phage delivery-related genes (lamB and malT) at lower bacterial concentration (twofold gene expression change). The top X-axis depicts decreasing bacterial concentration (CFU/mL). The expression level at a bacterial concentration of 108 CFU/mL was used as the control. Error bars depict ± one standard deviation from the mean of at least three independent replicates.
Plasmids Rp4 And R68.45, supplied by Federation of European Neuroscience Societies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+rp4/plasmids+rp4+and+r68+45/pm09570119-27-3-15
Average 90 stars, based on 1 article reviews
plasmids rp4 and r68.45 - by Bioz Stars, 2026-10
90/100 stars
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rp4: :xln hybrid plasmid  (Federation of European Neuroscience Societies)
90
Federation of European Neuroscience Societies rp4: :xln hybrid plasmid
Figure 2. Lower frequency but higher relative importance of genR transfer via phage delivery (relative to conjugation) occurs at lower bacterial concentration. (a) Transfer frequency was determined after 1 h of conjugation and phage delivery assays in minimal medium with 3.0 g/L glucose and shaken at an Re of 5 × 104. The genR donor (E. coli DH5α harboring plasmid <t>RP4</t> for conjugation assays or λ phage for phage delivery assays) and recipient (E. coli MG1655) were mixed at a 1:1 ratio. Frequency determined by the plate counting method is presented as bars with the detection limit (10−6) indicated by the horizontal dashed line; orange dots represent the frequency determined by qPCR analysis. Asterisks (*) indicate significant differences (p < 0.05) between conjugation and phage delivery frequency based on Student’s t-test. “N.D.” refers to “not detected”. (b) Upregulation of conjugation-related genes (traI, traJ, and trbAp) and phage delivery-related genes (lamB and malT) at lower bacterial concentration (twofold gene expression change). The top X-axis depicts decreasing bacterial concentration (CFU/mL). The expression level at a bacterial concentration of 108 CFU/mL was used as the control. Error bars depict ± one standard deviation from the mean of at least three independent replicates.
Rp4: :Xln Hybrid Plasmid, supplied by Federation of European Neuroscience Societies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+rp4/rp4+++xln+hybrid+plasmid/pm09742696-3-3-21
Average 90 stars, based on 1 article reviews
rp4: :xln hybrid plasmid - by Bioz Stars, 2026-10
90/100 stars
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plasmid rp4  (ACADEMIC PRESS INC)
90
ACADEMIC PRESS INC plasmid rp4
Figure 2. Lower frequency but higher relative importance of genR transfer via phage delivery (relative to conjugation) occurs at lower bacterial concentration. (a) Transfer frequency was determined after 1 h of conjugation and phage delivery assays in minimal medium with 3.0 g/L glucose and shaken at an Re of 5 × 104. The genR donor (E. coli DH5α harboring plasmid <t>RP4</t> for conjugation assays or λ phage for phage delivery assays) and recipient (E. coli MG1655) were mixed at a 1:1 ratio. Frequency determined by the plate counting method is presented as bars with the detection limit (10−6) indicated by the horizontal dashed line; orange dots represent the frequency determined by qPCR analysis. Asterisks (*) indicate significant differences (p < 0.05) between conjugation and phage delivery frequency based on Student’s t-test. “N.D.” refers to “not detected”. (b) Upregulation of conjugation-related genes (traI, traJ, and trbAp) and phage delivery-related genes (lamB and malT) at lower bacterial concentration (twofold gene expression change). The top X-axis depicts decreasing bacterial concentration (CFU/mL). The expression level at a bacterial concentration of 108 CFU/mL was used as the control. Error bars depict ± one standard deviation from the mean of at least three independent replicates.
Plasmid Rp4, supplied by ACADEMIC PRESS INC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+rp4/plasmid+rp4/pm06211681-19-14-31
Average 90 stars, based on 1 article reviews
plasmid rp4 - by Bioz Stars, 2026-10
90/100 stars
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rp4-tol cointegrate plasmid pww534  (ACADEMIC PRESS INC)
90
ACADEMIC PRESS INC rp4-tol cointegrate plasmid pww534
Figure 2. Lower frequency but higher relative importance of genR transfer via phage delivery (relative to conjugation) occurs at lower bacterial concentration. (a) Transfer frequency was determined after 1 h of conjugation and phage delivery assays in minimal medium with 3.0 g/L glucose and shaken at an Re of 5 × 104. The genR donor (E. coli DH5α harboring plasmid <t>RP4</t> for conjugation assays or λ phage for phage delivery assays) and recipient (E. coli MG1655) were mixed at a 1:1 ratio. Frequency determined by the plate counting method is presented as bars with the detection limit (10−6) indicated by the horizontal dashed line; orange dots represent the frequency determined by qPCR analysis. Asterisks (*) indicate significant differences (p < 0.05) between conjugation and phage delivery frequency based on Student’s t-test. “N.D.” refers to “not detected”. (b) Upregulation of conjugation-related genes (traI, traJ, and trbAp) and phage delivery-related genes (lamB and malT) at lower bacterial concentration (twofold gene expression change). The top X-axis depicts decreasing bacterial concentration (CFU/mL). The expression level at a bacterial concentration of 108 CFU/mL was used as the control. Error bars depict ± one standard deviation from the mean of at least three independent replicates.
Rp4 Tol Cointegrate Plasmid Pww534, supplied by ACADEMIC PRESS INC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+rp4/rp4+tol+cointegrate+plasmid+pww534/pm01513877-16-2-26
Average 90 stars, based on 1 article reviews
rp4-tol cointegrate plasmid pww534 - by Bioz Stars, 2026-10
90/100 stars
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rp4-11 (plasmid #79812)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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rp4-6 (plasmid #80385)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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Image Search Results


Figure 2. Lower frequency but higher relative importance of genR transfer via phage delivery (relative to conjugation) occurs at lower bacterial concentration. (a) Transfer frequency was determined after 1 h of conjugation and phage delivery assays in minimal medium with 3.0 g/L glucose and shaken at an Re of 5 × 104. The genR donor (E. coli DH5α harboring plasmid RP4 for conjugation assays or λ phage for phage delivery assays) and recipient (E. coli MG1655) were mixed at a 1:1 ratio. Frequency determined by the plate counting method is presented as bars with the detection limit (10−6) indicated by the horizontal dashed line; orange dots represent the frequency determined by qPCR analysis. Asterisks (*) indicate significant differences (p < 0.05) between conjugation and phage delivery frequency based on Student’s t-test. “N.D.” refers to “not detected”. (b) Upregulation of conjugation-related genes (traI, traJ, and trbAp) and phage delivery-related genes (lamB and malT) at lower bacterial concentration (twofold gene expression change). The top X-axis depicts decreasing bacterial concentration (CFU/mL). The expression level at a bacterial concentration of 108 CFU/mL was used as the control. Error bars depict ± one standard deviation from the mean of at least three independent replicates.

Journal: ACS environmental Au

Article Title: Bacterial Concentrations and Water Turbulence Influence the Importance of Conjugation Versus Phage-Mediated Antibiotic Resistance Gene Transfer in Suspended Growth Systems.

doi: 10.1021/acsenvironau.1c00027

Figure Lengend Snippet: Figure 2. Lower frequency but higher relative importance of genR transfer via phage delivery (relative to conjugation) occurs at lower bacterial concentration. (a) Transfer frequency was determined after 1 h of conjugation and phage delivery assays in minimal medium with 3.0 g/L glucose and shaken at an Re of 5 × 104. The genR donor (E. coli DH5α harboring plasmid RP4 for conjugation assays or λ phage for phage delivery assays) and recipient (E. coli MG1655) were mixed at a 1:1 ratio. Frequency determined by the plate counting method is presented as bars with the detection limit (10−6) indicated by the horizontal dashed line; orange dots represent the frequency determined by qPCR analysis. Asterisks (*) indicate significant differences (p < 0.05) between conjugation and phage delivery frequency based on Student’s t-test. “N.D.” refers to “not detected”. (b) Upregulation of conjugation-related genes (traI, traJ, and trbAp) and phage delivery-related genes (lamB and malT) at lower bacterial concentration (twofold gene expression change). The top X-axis depicts decreasing bacterial concentration (CFU/mL). The expression level at a bacterial concentration of 108 CFU/mL was used as the control. Error bars depict ± one standard deviation from the mean of at least three independent replicates.

Article Snippet: Conjugative plasmid RP4 was obtained from Addgene (plasmid 79813).

Techniques: Conjugation Assay, Concentration Assay, Plasmid Preparation, Gene Expression, Expressing, Control, Standard Deviation

Figure 3. Turbulence (represented by Reynolds number) significantly impacts the relative frequency of conjugation vs phage delivery for the horizontal transfer of genR in a bell-shaped fashion. The transfer frequency was determined after 1 h of conjugation and phage delivery assays in minimal medium containing 3.0 g/L glucose with bacterial concentration at 107 CFU/mL. The genR donor (E. coli DH5α harboring plasmid RP4 for conjugation assays or λ phage for phage delivery assays) and recipient (E. coli MG1655) were mixed at a 1:1 ratio. Asterisks (*) indicate significant differences (p < 0.05) between conjugation and phage delivery frequency based on Student’s t-test. Error bars depict ± one standard deviation from the mean of at least three independent replicates.

Journal: ACS environmental Au

Article Title: Bacterial Concentrations and Water Turbulence Influence the Importance of Conjugation Versus Phage-Mediated Antibiotic Resistance Gene Transfer in Suspended Growth Systems.

doi: 10.1021/acsenvironau.1c00027

Figure Lengend Snippet: Figure 3. Turbulence (represented by Reynolds number) significantly impacts the relative frequency of conjugation vs phage delivery for the horizontal transfer of genR in a bell-shaped fashion. The transfer frequency was determined after 1 h of conjugation and phage delivery assays in minimal medium containing 3.0 g/L glucose with bacterial concentration at 107 CFU/mL. The genR donor (E. coli DH5α harboring plasmid RP4 for conjugation assays or λ phage for phage delivery assays) and recipient (E. coli MG1655) were mixed at a 1:1 ratio. Asterisks (*) indicate significant differences (p < 0.05) between conjugation and phage delivery frequency based on Student’s t-test. Error bars depict ± one standard deviation from the mean of at least three independent replicates.

Article Snippet: Conjugative plasmid RP4 was obtained from Addgene (plasmid 79813).

Techniques: Conjugation Assay, Concentration Assay, Plasmid Preparation, Standard Deviation